KMID : 1134820160450050690
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Journal of the Korean Society of Food Science and Nutrition 2016 Volume.45 No. 5 p.690 ~ p.695
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Characterization of Styela clava Tunic after Alkaline Treatment
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Kim Min-Jeong
KimWon-Baek Hwang Ji-Hoe Kim Su-Ae Kim Bo-Ram Koo Kyoung-Yoon Son Hong-Joo Hwang Dae-Yon Jung Young-Jin Lee Hee-Seob
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Abstract
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This study was performed to evaluate the effect of alkaline treatment on Styela clava tunic (SCT). Considerable damage to the surface of alkali-treated SCT was observed by scanning electron microscopy (SEM) in a concentration-dependent manner upon alkaline treatment. The amount of crystalline region in SCT gradually increased with increasing NaOH concentration, which was analyzed by X-ray diffraction and thermogravimetric analysis. The initial enzymatic reaction of Celluclast toward SCT was elevated by treatment with NaOH up to 1.0 N concentration due to disruption of the SCT surface by promoting binding of enzymes with SCT. However, in the late stage of the enzyme reaction, hydrolysis rate decreased with elevation of NaOH concentration, thereby increasing the amount of non-reacted residuals. This result was due to the increase in the crystalline regions in SCT.
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KEYWORD
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Styela clava tunic (SCT), alkaline treatment, crystallinity, cellulose, cellulase digestibility
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